To mitigate the problems of long working time, low efficiency and large manpower investment in the test of one key sequence control, the one key sequence control specification of the new?generation autonomous and controllable substation proposes the non?outage verification method. The sequence control operation ticket test can be accomplished by the simulation of the main and auxiliary integrated monitoring host, which can solve the above problems. A one key sequence control test method for new?generation autonomous and controllable substation is proposed. The configuration information points required by the one key sequence control test library are formed by constructing an intermediate database based on the signal definition in the standard one key sequence control operation ticket. Based on the standard one key sequence control operation ticket and the interval definition under typical wiring mode, the one key sequence control test library is constructed by using the information points in the intermediate database. Based on the optimized TF?IDF weighted text semantic similarity algorithm, the instantiation of the test library is realized. The test library is applied to the one key sequence control simulation test in specific projects to solve the one key sequence control simulation test problem. The effectiveness of the proposed method is verified by an actual simulation test example.